Identification of Bacteria in Dental Plaque

Syahdiana Waty, Nurul Hidayah

Abstract


Oral health has an important role in overall body health. This study aims to evaluate the antibacterial efficacy of toothpaste with cinnamon ethanol extract against Streptococcus bacteria in vivo, analyze changes in the number of bacterial colonies before and after using toothpaste, and identify the type of Streptococcus bacteria in dental plaque. This pure experimental research was conducted at the Pharmacy Laboratory of the Poltekkes Kemenkes Medan and the Microbiology Laboratory of the Faculty of Medicine, University of North Sumatra. Samples included cinnamon extract, toothpaste formulation with 12.5% extract concentration, and dental plaque from 40 respondents. Data analysis used descriptive univariate test with SPSS 22.0 application. Results showed Gram-positive bacterial infections, especially Rothia dentocariosa (24%), dominated dental plaque, followed by Streptococcus mitis (16.4%). The use of cinnamon-active toothpaste was able to significantly reduce the number of bacterial colonies, especially at high concentrations. In addition, active compounds in cinnamon extracts such as cinnamaldehyde and eugenol showed the ability to damage bacterial cell walls and inhibit biofilm formation. Gargling was also shown to be effective in reducing bacterial colonies by clearing plaque and food debris. The conclusion of this study suggests that Gram-positive bacterial infections in dental plaque require special attention in management with appropriate antibiotics, while gargling and the use of cinnamon-based antimicrobial agents can be an effective preventive strategy. These findings support the development of natural ingredient-based oral health products to improve infection prevention and the quality of oral health care.

 

Keywords: Oral Health, Streptococcus, Cinnamon, Antibacterial, Dental Plaque, Gargling

Full Text:

PDF

References


Akanchi, M., Ashayeri, H., Mottaghi, Z., & Golmohammadi, M. (2022). Systematic Investigations of the Healing Process of Skin, Oral and Dental Wounds and Cardiac and Pulmonary Complications and Drug Therapy in Patients with Infectious Diseases. NeuroQuantology, 20(8), 3015. https://doi.org/10.14704/nq.2022.20.8.NQ44334

Baquero, F., Saralegui, C., Marcos-Mencía, D., Ballestero, L., Vañó-Galván, S., Moreno-Arrones, Ó. M., & del Campo, R. (2021). Epidermis as a Platform for Bacterial Transmission. Frontiers in Immunology, 12(December), 1–11. https://doi.org/10.3389/fimmu.2021.774018

Bessa, L. J., Botelho, J., Machado, V., Alves, R., & Mendes, J. J. (2022). Managing Oral Health in the Context of Antimicrobial Resistance. International Journal of Environmental Research and Public Health, 19(24). https://doi.org/10.3390/ijerph192416448

Born, C. D., Jackson, T. H., Koroluk, L. D., & Divaris, K. (2019). Traumatic dental injuries in preschool-age children: Prevalence and risk factors. Clinical and Experimental Dental Research, 5(2), 151–159. https://doi.org/10.1002/cre2.165

Cebrián, G., Condón, S., & Mañas, P. (2017). Physiology of the inactivation of vegetative bacteria by thermal treatments: Mode of action, influence of environmental factors and inactivation kinetics. Foods, 6(12), 1–21. https://doi.org/10.3390/foods6120107

Chokshi, A., Sifri, Z., Cennimo, D., & Horng, H. (2019). Global contributors to antibiotic resistance. Journal of Global Infectious Diseases, 11(1), 36–42. https://doi.org/10.4103/jgid.jgid_110_18

Didehdar, M., Chegini, Z., Tabaeian, S. P., Razavi, S., & Shariati, A. (2022). Cinnamomum: The New Therapeutic Agents for Inhibition of Bacterial and Fungal Biofilm-Associated Infection. Frontiers in Cellular and Infection Microbiology, 12(July), 1–19. https://doi.org/10.3389/fcimb.2022.930624

Do, H., Makthal, N., VanderWal, A. R., Saavedra, M. O., Olsen, R. J., Musser, J. M., & Kumaraswami, M. (2019). Environmental pH and peptide signaling control virulence of Streptococcus pyogenes via a quorum-sensing pathway. Nature Communications, 10(1). https://doi.org/10.1038/s41467-019-10556-8

Elcocks, E. R., Spencer-Phillips, P. T. N., & Adukwu, E. C. (2020). Rapid bactericidal effect of cinnamon bark essential oil against Pseudomonas aeruginosa. Journal of Applied Microbiology, 128(4), 1025–1037. https://doi.org/10.1111/jam.14538

Firmansyah, R., & Lingga, F. D. P. (2024). UJI DAYA HAMBAT EKSTRAK KULIT KAYU MANIS TERHADAP PERTUMBUHAN JAMUR TRICHOPHYTON MENTAGROPHYTES SECARA IN VITRO. Jurnal Ilmiah Maksitek, 9(1), 60–68. https://doi.org/10.1101/2020.11.10.376129

Freiberg, A., Wienke, A., Bauer, L., Niedermaier, A., & Führer, A. (2020). Dental care for asylum-seekers in germany: A retrospective hospital-based study. International Journal of Environmental Research and Public Health, 17(8). https://doi.org/10.3390/ijerph17082672

Haque, M., Sartelli, M., & Haque, S. Z. (2019). Dental infection and resistance-global health consequences. Dentistry Journal, 7(1), 1–19. https://doi.org/10.3390/dj7010022

Hescot, P. (2017). The New Definition of Oral Health and Relationship between Oral Health and Quality of Life. The Chinese Journal of Dental Research : The Official Journal of the Scientific Section of the Chinese Stomatological Association (CSA), 20(4), 189–192. https://doi.org/10.3290/j.cjdr.a39217

Inchingolo, A. D., Malcangi, G., Semjonova, A., Inchingolo, A. M., Patano, A., Coloccia, G., Ceci, S., Marinelli, G., Di Pede, C., Ciocia, A. M., Mancini, A., Palmieri, G., Barile, G., Settanni, V., De Leonardis, N., Rapone, B., Piras, F., Viapiano, F., Cardarelli, F., … Dipalma, G. (2022). Oralbiotica/Oralbiotics: The Impact of Oral Microbiota on Dental Health and Demineralization: A Systematic Review of the Literature. Children, 9(7). https://doi.org/10.3390/children9071014

Intan, K., Diani, A., & Nurul, A. S. R. (2021). Aktivitas Antibakteri Kayu Manis (Cinnamomum burmanii) terhadap Pertumbuhan Staphylococcus aureus. JURNAL KESEHATAN PERINTIS (Perintis’s Health Journal), 8(2), 121–127. https://doi.org/10.33653/jkp.v8i2.679

Jakubovics, N. S., Goodman, S. D., Mashburn-Warren, L., Stafford, G. P., & Cieplik, F. (2021). The dental plaque biofilm matrix. Periodontology 2000, 86(1), 32–56. https://doi.org/10.1111/prd.12361

Jans, C., & Boleij, A. (2018). The road to infection: Host-microbe interactions defining the pathogenicity of Streptococcus bovis/Streptococcus equinus complex members. Frontiers in Microbiology, 9(APR). https://doi.org/10.3389/fmicb.2018.00603

Juwita Husaini, Rosmawaty, Muhammad Tahir, & Ayu Lestari. (2024). Decreased Plaque Score Due to Gargling with Guava Leaf Decoction Among Adolescents at the “Sejati Muhammadiyah Rappang” Social Welfare Institution Sidenreng Rappang Regency. Journal of Community Engagement in Health, 7(1), 50–57. https://doi.org/10.30994/jceh.v7i1.526

Kemenkes RI. (2018). Laporan Nasional Riset Kesehatan Dasar. In Badan Penelitian dan Pengembangan Kesehatan (p. 674).

Komala, O., Noorlaela, E., & Dhiasmi, A. (2018). UJI ANTIBAKTERI DAN FORMULASI SEDIAAN MASKER ANTI JERAWAT YANG MENGANDUNG KAYU MANIS (Cinnamomum burmanni Nees &T. Nees ). Ekologia, 18(1), 31–39.

Lee, Y. H. (2022). Supportive Home Remedies for Orofacial Pain during the Coronavirus Disease 2019 Pandemic: Their Value and Limitations. International Journal of Dentistry, 2022. https://doi.org/10.1155/2022/2005935

Li, J., Xie, S., Ahmed, S., Wang, F., Gu, Y., Zhang, C., Chai, X., Wu, Y., Cai, J., & Cheng, G. (2017). Antimicrobial activity and resistance: Influencing factors. Frontiers in Pharmacology, 8(JUN), 1–11. https://doi.org/10.3389/fphar.2017.00364

Loban, G. A., Faustova, M. O., Chereda, V. V., & Ananieva, M. M. (2021). Epidemiological and etiological aspects of dental caries development. Acta Facultatis Medicae Naissensis, 38(1), 27–34. https://doi.org/10.5937/AFMNAI38-27564

Machado, S. G., Baglinière, F., Marchand, S., Coillie, E. Van, Vanetti, M. C. D., Block, J. De, & Heyndrickx, M. (2017). The biodiversity of the microbiota producing heat-resistant enzymes responsible for spoilage in processed bovine milk and dairy products. Frontiers in Microbiology, 8(MAR), 1–22. https://doi.org/10.3389/fmicb.2017.00302

Mishra, A., Nam, G. H., Gim, J. A., Lee, H. E., Jo, A., & Kim, H. S. (2018). Current challenges of Streptococcus infection and effective molecular, cellular, and environmental control methods in aquaculture. Molecules and Cells, 41(6), 495–505. https://doi.org/10.14348/molcells.2018.2154

Ng, L., Malandris, M., Cheung, W., & Rossi-Fedele, G. (2020). Traumatic dental injuries presenting to a paediatric emergency department in a tertiary children’s hospital, Adelaide, Australia. International Journal of Dentistry, 36(4), 360–370. https://doi.org/https://doi.org/10.1111/edt.12548

Nuriyah, E., Edi, I. S., & Ulfah, S. F. (2022). Karies Gigi Ditinjau dari Pengetahuan Kesehatan Gigi dan Mulut pada Siswa Sekolah Dasar. Indonesian Journal of Health and Medical , 2(2), 167–179.

Parhusip, A. J. N., & Cynthia, L. J. (2019). Aplikasi Ekstrak Kulit Kayu Manis (Cinnamomum burmanii) untuk Menghambat Aktivitas Bakteri Ikan Lele (Clarias batrachus). Jurnal Sains Dan Teknologi, 3(2), 66–84.

Petti, S., Glendor, U., & Andersson, L. (2018). World traumatic dental injury prevalence and incidence, a meta-analysis—One billion living people have had traumatic dental injuries. Dental Traumatology, 34(2), 71–86. https://doi.org/10.1111/edt.12389

Putri, P. P., Sahidan, S., & Susiwati, S. (2023). Gambaran Daya Hambat Ekstrak Kulit Kayu Manis (Cinnamomum Burmannii Blume) Terhadap Pertumbuhan Bakteri Staphylococcus Aureus. Jurnal Fatmawati Laboratory & Medical Science, 3(1), 28–34. https://doi.org/10.33088/flms.v3i1.406

S S, N., P Puranik, M., & K R, S. (2022). Added sugars and their impact on dental caries: A narrative review. RGUHS Journal of Dental Sciences, 14(4), 2–7. https://doi.org/10.26463/rjds.14_4_21

Santamaria, R. M., Schmoeckel, J., Basner, R., Schüler, E., & Splieth, C. H. (2019). Caries Trends in the Primary Dentition of 6- to 7-Year-old Schoolchildren in Germany from 1994 to 2016: Results from the German National Oral Health Surveys in Children. Caries Research, 53(6), 659–666. https://doi.org/10.1159/000500854

Saputri, D. Y., Hadi, S., & Marjianto, A. (2022). Hubungan Cara Menyikat Gigi Dengan Karies Gigi Pada Siswa Kelas XI SMA Widya Darma Surabaya. E-Indonesian Journal of Health and Medical, 2(3), 233–243. http://ijohm.rcipublisher.org/index.php/ijohm

Septiani, D., Sughesti, D., Susanti, D., Sihombing, M. T. P., & Novitasari, S. (2021). Pentingnya Menjaga Kesehatan Gigi Dan Mulut Di Era Pandemi Covid’19, Demi Kelangsungan Aktivitas Usaha. Dedikasi Pkm, 3(1), 56. https://doi.org/10.32493/dedikasipkm.v3i1.14607

Siregar, R. A., Lestari, I. C., Rangkuti, I. Y., & Sari, S. K. (2023). Uji Efektivitas Antibiotik Ekstrak Etanol Daun Kayu Manis (Cinnamomum Burmannii) Terhadap Staphylococcus Aureus Secara in Vitro. Jurnal Kedokteran STM (Sains Dan Teknologi Medik), 6(2), 143–150. https://doi.org/10.30743/stm.v6i2.389

Syahdiana Waty, & Nurul Hidayah. (2023). Uji Toksisitas Akut Ekstrak Etanol Kayu Manis. Jurnal Ilmiah PANNMED (Pharmacist, Analyst, Nurse, Nutrition, Midwivery, Environment, Dentist), 18(1), 131–136. https://doi.org/10.36911/pannmed.v18i1.1563

Wang, S. P., Rubio, L. A., Duncan, S. H., Donachie, G. E., Holtrop, G., Lo, G., Farquharson, F. M., Wagner, J., Parkhill, J., Louis, P., Walker, A. W., & Flint, H. J. (2020). Pivotal Roles for pH, Lactate, and Lactate-Utilizing Bacteria in the Stability of a Human Colonic Microbial Ecosystem. MSystems, 5(5). https://doi.org/10.1128/msystems.00645-20

Waty, S., Suryanto, D., & Yurnaliza. (2018). Antibacterial activity of cinnamon ethanol extract (cinnamomum burmannii) and its application as a mouthwash to inhibit streptococcus growth. IOP Conference Series: Earth and Environmental Science, 130(1). https://doi.org/10.1088/1755-1315/130/1/012049




DOI: http://dx.doi.org/10.30829/contagion.v7i1.21578

Refbacks

  • There are currently no refbacks.


Copyright (c) 2025 Syahdiana Waty, Nurul Hidayah

Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

 
Contagion: Scientific Periodical Journal of Public Health and Coastal Health by Program Studi Ilmu Kesehatan Masyarakat is licensed under Creative Commons Attribution-ShareAlike 4.0 International License.