Potential Antifungal of Merang Padi Extract (Oryza sativa) Against Fungal Growth

Masniah Masniah, Ahmad Purnawarman Faisal

Abstract


Merang padi is a traditional medicine that comes from natural ingredients, namely plants. However, there has not been much research on the activity of rice straw extract (Oryza sativa) being explored and researched. The aim of this research was to determine the yield of extracts and the number of antifungal isolates in inhibiting fungal growth and their potential as antifungals against Candida albicans and Aspergillus fumigatus fungi. This research uses experimental research with a quantitative approach carried out through laboratory tests. The design used was a Completely Randomized Design (CRD) which was grouped into 6 treatment groups, namely 4 groups of rice straw extract and 2 control groups which were repeated for each treatment 4 times. The treatment group consisted of P1 with rice straw extract with a concentration of 20,000 ppm, P2 with rice straw extract with a concentration of 40,000 ppm, P3 with rice straw extract with a concentration of 60,000 ppm and P4 with rice straw extract with a concentration of 80,000 ppm. Meanwhile, the control group, namely P0 as a negative control, namely NaCl 0.9% and P5 as a positive control, namely Nystatin 100,000 IU. The research sample was rice straw from rice plants that had been identified and then weighed. Data analysis used one way ANOVA SPSS version 20. If there were differences then a further test was carried out, namely the Honest Significant Difference Test (BNJ) to see the differences between each treatment based on the coefficient of diversity (KK) value obtained at the 5% test level. The results of tests that have been carried out show that the n-hexane fraction of rice straw has no antifungal activity, while the ethyl acetate and n-butanol fractions have antifungal activity. The concentration of ethyl acetate in this study was greater than the other compounds.

 

Keywords : Candida albicus, merang padi, mushrooms, oryza sativa


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References


Aly, M. M., Al-Aidroos, B. A., & Alfassi, F. A. (2011). Production of non polyenic antifungal agent from Streptomyces sp BM54, isolated from marine shripms. Crown Journal of Medicine, 1(1), 01–08.

Anggraeni, P. P., Chatri, M., & Advinda, L. (2023). Characteristics of Saponin Secondary Metabolite Compounds in Plants Karakteristik Saponin Senyawa Metabolit Sekunder pada Tumbuhan. Serambi Biologi, 8(2), 251–258.

Balouiri, M., Moulay, S., & Saad, K. I. (2016). Methods for in vitro evaluating antimicrobial activity: A review. Journal of Pharmaceutical Analysis, 6, 71–79.

Hamidah. (2013). Isolasi dan identifikasi isolat actinomycetes dari rizosfer padi. Skripsi. http://eprints.ums.ac.id/24203/1/COVER-INTISARI.pdf

Hasanah, U. (2017). Mengenal Aspergillosis, Infeksi Jamur Genus Aspergillus. Jurnal Keluarga Sehat Sejahtera, 15(2), 76–86. https://doi.org/10.24114/jkss.v15i2.8777

Hasim, S., & Coleman, J. J. (2019). Targeting the fungal cell wall: Current therapies and implications for development of alternative antifungal agents. Future Medicinal Chemistry, 11(8), 869–883. https://doi.org/10.4155/fmc-2018-0465

Hidayah, N., Mustikaningtyas, D., & Bintari, S. H. (2017). Aktivitas Antibakteri Infusa Simplisia Sargassum muticum terhadap Pertumbuhan Staphylococcus aureus. Life Science, 6(2), 49–54. http://journal.unnes.ac.id/sju/index.php/UnnesJLifeSci

Hsu, P., Bullocks, J., & Matthews, M. (2006). Infection Prophylaxis Update. Seminars in Plastic Surgery, 20(4), 241–248. https://doi.org/10.1055/s-2006-951582

Ihwana, H., Efendi Hutagalung, J., Sistem Informasi, P., & Tinggi Manajemen Informatika Royal Kisaran, S. (2022). Diagnosis of Human Skin Fungi Using Fordward Chaning Method Based On. Jurnal Teknik Informatika (JUTIF), 3(3). https://doi.org/10.20884/1.jutif.2022.3.3.233

Indriani, C., FR, F., & L, K. (2020). Identification Of Aspergillus Sp Growth On White Bread Against Storage Temperature. Jurnal Kesehatan Rajawali, 10(2), 92–103.

Julianto, T. S. (2019). Fitokimia Tinjauan Metabolit Sekunder dan Skrining fitokimia. In Jakarta penerbit buku kedokteran EGC (Vol. 53, Issue 9).

Kumamoto, C. A., Gresnigt, M. S., & Hube, B. (2021). Commensal and Opportunistic Pathogen in the Intestine. 7–15. https://doi.org/10.1016/j.mib.2020.05.006.The

Maisarah, M., Chatri, M., & Advinda, L. (2023). Karakteristik dan Fungsi Senyawa Alkaloid sebagai Antifungi pada Tumbuhan. Journal Serambi Biologi, 8(2), 231–236.

Mirza, S. A., Phelan, M., Rimland, D., Graviss, E., Hamill, R., & Brandt, M. E. (2003). The changing epidemiology of cryptococcosis: an update from population-based active surveillance in 2 large metropolitan areas. Clinical Infection Diseases, 36, 789–794.

Mutiawati, V. K. (2016). Pemeriksaan Mikrobiologi pada Candida Albicans. Jurnal Kedokteran Syiah Kuala, 97(1), 53–63. https://doi.org/10.1016/s0035-9203(03)90055-1

Pelczar, M. J., & Chan, E. C. S. (2007). Dasar-Dasar Mikrobiologi (Jilid I). Jakarta : UI Press.

Rohmawati, E. S., & Harahap, I. (2017). Isolasi dan karakterisasi senyawa antifungi isolat cendawan endofit dari tumbuhan senduduk (Melastoma malabathricum L.). Prosiding Celscitech-Umri 2017, 2, 43–49.

Romanos, M. A., Scorer, C. A., & Clare, J. J. (1992). Foreign Gene Expression in Yeast. 8, 423–488.

Salmiati, T., Arwie, D., & Fatimah. (2021). Identifikasi Candida Albicans Pada Saliva Penderita Diabetes Melitus Di Wikayah Kerja Puskesmas Gattareng. Jurnal TLM Blood Smear, 2(1), 26–30. https://doi.org/10.37362/jmlt.v2i1.426

Septiadi, T., Pringgenies, D., & Radjasa, O. K. (2013). Uji Fitokimia dan Aktivitas Antijamur Ekstrak Teripang Keling (Holoturia atra) dari Pantai Bandengan Jepara terhadap Jamus Candida Albicans. Journal of Marine Research, 2(2), 76–84.

Siregar, S. I. S., Oktarina, H., Hakim, L., Tanaman, J. P., Pertanian, F., & Kuala, U. S. (2022). Tanaman Padi Yang Berpotensi Sebagai Agens Pengendali Hayati Penyakit Blas ( Pyricularia oryzae ) Pada Padi ( Exploration and Identification of Endophytic Fungus from Padi Plants : study on Potential Biological Control Agents of Blast Disease Pyricularia. Jurnal Ilmiah Mahasiswa Pertanian, 7(1), 749–760.

Suraini, S. dan. (2023). Analisa Jamur Candida albicans Pada Swab Mukosa Mulut Perokok Aktif di Lubuk Buaya. Jurnal Biologi Makassar, 8, 31–38.

Suriani, R. (2018). Formulasi Sedian Shampo dari Merang Padi ( Oryza sativa ). Tugas Akhir.

Triana, O., Prasetya, F., Kuncoro, H., & Rijai, L. (2016). Aktivitas Antijamur Ekstrak Daun Ketepeng Cina (Cassia alata L.). Jurnal Sains Dan Kesehatan, 1(6), 311–315.

WHO. (2009). Laboratory Manual for Diagnosis of Fungal Opportunistic Infections in HIV/AIDS Patients.




DOI: http://dx.doi.org/10.30829/contagion.v6i1.19288

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