Isolasi Antimikroba Baru dari Bakteri Tanah

Evi Damayanti, Firdaus Hamid, Rizalinda Sjahril

Abstract


Antimicrobial resistance has become a problem and a threat to health both in Indonesia and in the world. Resistance is the ability of bacteria to neutralize and weaken the action of antibiotics. The aim of this study was to isolate a novel antimicrobial from soil bacteria. The method used in this research is exploratory. The test bacteria used were Staphylococcus aureus and Pseudomonas aeruginosa. The results showed that there were two isolates that had zones of inhibition against the test bacteria staphylococcus aureus, namely isolates T2.2 and T2.18. The results of the first antimicrobial test for isolate T2.2 showed that the inhibition zone formed was 14.05 mm and isolates T2.18 the inhibition zone formed was 11.96 mm. The results of the second antimicrobial test were carried out by treating the unheated T2.2 isolate which showed an inhibition zone formed of 15.53 mm and the heated one 15.46 mm, while the unheated T2.18 isolate showed an average inhibition zone of 12.46 mm and heated 12.21 mm. The conclusion of this study is that the inhibition zone formed on isolates T2.2 and T2.18 is still relatively small and both isolates are spesies of Pseudomonas aeruginosa.

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References


Antonio R, Mohidra Seeprsaud, Anderson Maxweel, Jayaraj jayaraman, and Adesh Ramsubhag. 2020. Isolation and Antibacterial Activity of Indole Alkaloids from Pseudomonas aeruginosa UWE-1. University of The West Indies.

Asmira Azisa Nur. 2013. Isolasi Antimikroba Penghasil Antibiotika dari Tanah Sekitar Pembuangan Limbah Pabrik Gula Takalar. Universitas Hasanuddin Makassar.

CDC. Antibiotic Use in the United states, 2018 Update: Progres and opportunities. Atlanta, GA: US Departement of Health and Human Services, CDC;2019

Elena Trizna Y., Maria N. Yarullina., Diana R. Baidamashina, et al. 2020. Bidirectional alterations in antibiotics susceptibility ini Staphylococcusaureus-Pseudomonas aeruginosa dual species biofilm. Kazan federal University.

Hadi U, et al. 2008. Audit of Antibiotic Prescribing in Two Govermental Teaching Hospital in Indonesia. Clinical Microbiology and Infection : the official of the European Society Clinical Microbiology and Infection. : the official of the European Society Clinical Microbiology and Infection Disease, 14 (7):698-707.DOI:Available at: https://doi.org/10.1111/j.1469- 0691.2008.02014.x.

Ivoryanto E.,Sidarta B.,Illahi K. (2017). Hubungan tingkat pendidikan formal masyarakat terhadap pengetahuan dalam penggunaan antibiotika oral di Apotek Kecamatan Klojen. Pharmaceutical Journal of Indonesia. 2(2);31-6.

Kementrian Kesehatan Republik Indonesia. Riset Kesehatan Dasar (RISKESDAS). Jakarta, Indonesia: Kemenkes. 2013. Hal 40-7.

Lauren Radlinski., Sarah E., Laurel B. K. et al. 2017. Pseudomonas aeruginosa exoproducts determine antibiotic efficacy against Staphylococcus aureus. University of North Carolina at Chapel Hill, North Carolina United States of America.

Ling-Qing Xu, Jian-Wen Zeng, Chong-He Jiang. Et al. 2017. Isolation and determination of four potential antimicrobial components from Pseudomonas aeruginosa extracts. Lyspring International Publisher.

Suarez, C and Gudiol, F. 2009. Beta-lactam antibiotics. Enfermedades infecciosas ymicrobiologia clinica. 27(2). 116-129.

Sukmawati dan Febrianti Rosalina. 2020. Isolasi Antimikroba dari Tanah Sebagai Penghasil Senyawa. Universitas Muhammadiyah Sorong,

Utami, rahayu. 2011. Antibiotik, Resistensi dan Rasionalisasi Terapi. 1(4): 191-8.

Van Veen, S. Q.,E. C. Class and E. J. Kuijper. 2010. High-throughput inditification of bacteria and yeast by matrix assisted laser desorption ionization-time of flight mass spectrometry in conventional medical microbiology laboratories. J. Clin. Microbiol. 48:900-907.

Wardhani Aliya. 2018. Perbandingan hasil identifikasi yeast dengan menggunakan biokimia otomatis (ViteksĀ® 2) dan MALDI-TOF MS (ViteksĀ® MS). Universitas Sebelas Maret.

Yulia R., Putri R., Wahyudi R. 2019. Studi Tingkat Pengetahuan Masyarakat Terhadap Penggunaan Antibiotik di Puskesmas Rasimah Ahmad Bukittinggi. Journal of Pharmaceceutical and Sciences (JPS). 2(2): 43-8.

Zina Nasfi.,Hendrik Buch.,Stefan Kehraus.,et al. 2018. Soil Bacteria Isolated From Tunisian Arid Areas Show Promising Antimicrobial Activities Against Gram-Negatives. Laboratory of Plant Improvement and Valorization of Agroresources, National School of Engineering of Sfax. Tunisia.




DOI: http://dx.doi.org/10.30829/jumantik.v7i2.11266

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